Mycoplasma research

Mycoplasma Research Department

 Specializes in the following:

Quick examination using real-time serial polymerase enzyme testing

Examination using random amplification of DNA to differentiate between strains

Field and other used for immunization.

Isolation and classification of mycoplasma by conventional methods.

Do an allergy test for isolates to choose the most appropriate antibiotic

Used for treatment with the lowest effective concentration method (MIC).

Serological examination of the serum of infected or immunized birds by ELISA and hemodynamic (SPA) tests.

Evaluation of the immune level of birds immunized with mycoplasma vaccines.

Mycology Research

Fungi Research Department

Specializes in the following:

Examination of sick animals and sick and dead birds suspected of infection with various fungi, isolation and classification of pathogenic fungi.

Examining the diets and their components, isolating the contaminated fungi, and determining their toxicity and the viability of these diets for storage or consumption.

Serological diagnosis of fungal diseases and classification of some pathological types of fungi.

Preparing diagnostic materials for some fungal diseases.

Conducting research on fungal diseas

Poultry Diseases Research

Poultry Diseases Research Department

Specializes in the following:

Isolating and classifying viruses that infect birds by injecting eggs into different embryos according to the type of each virus.

Measuring the immune bodies in the blood of birds for viral diseases.

Diagnosis of external parasites that affect birds and rabbits or their whereabouts.

Diagnosis of internal and primary parasites in birds and glaucoma.

Examination of samples of fertilized eggs and embryos for different poultry

Isolating the causes of epidemic, infectious and bacterial diseases, especially Salmonella.

Perform the necessary biochemical and serological tests to classify the isolated microbes.

A bacterial examination of the disease states of the birds (sick or dead) and rabbits

Firmness tests for different isolates by injection into experimental animals.

The anatomical characteristic of poultry and rabbits mentioned in the department.

Performing a histopathological examination on the infected or suspected organ.

Diagnosis of cancerous diseases in poultry.

Fish diseases research

Fish diseases research department

Specializes in the following:

Examination of fish fry and fingerlings used for fish farming before any fish project is started to ensure that they are free of pathogens.

Periodic examination of the various fish farming projects to ensure that they are free of pathogens during the period of the productive cycle and to diagnose and treat any pathological problems if any.

Examination of water samples to ensure their suitability with international standards, to ensure their suitability for fish farming, to provide solutions to their problems, and to advise the customer of the best types of fish suitable for culture.

Microbiological examination of water samples, determination of the total bacterial count, the count of intestinal bacteria and their suitability for fish culture.

A complete blood test for fish and marine mammals found in waterways in Egypt.

Chemical examination of serum components, enzymes and hormones

The ability to examine fish to detect various toxins that are transmitted to humans.

Assisting clients of fish farms in making aquarium fish tanks and providing advice and guidance to the best and easiest species in hatchery.

Examination of fry, farm fish, ornamental fish, chimneys, crustaceans and molluscs for parasitic, bacterial and fungal pathogens and ensuring that they are free of infectious, epidemic and common diseases in accordance with local and international specifications, using the latest modern devices and advanced technologies.

Examine frozen and smoked fish, crustaceans, mollusks, fish oils, fish feed, cat food and imported dogs prepared for export for bacterial, fungal and parasitic pathogens, and ensure that they are free of infectious, epidemic and common diseases in accordance with local and international specifications, using the latest modern devices and advanced technologies.

Periodic monitoring of all environmental factors required for fish farming purposes before and during project operation.

Measuring the fertility rate in fish to determine the breeding seasons.

Examination of gonads of live and frozen fish to isolate pathogens of parasitic, bacterial and fungal diseases that affect fish fertility and reduce their productivity and try to treat them.

Parasitology Research

Parasitology Research Department

ٍٍSpecializes in the following:

Examination of horse serum for the pyroplasmosis parasite and syphilis using Elisa.

Examination of blood and tissue samples to detect parasites, papillas, thylaria, anaplasma, trypanosoma and microfilaria.

Injecting laboratory animals to diagnose the trypanosome parasite.

Examination of animal skins and skins for external parasites and their phases.

Examination and classification of external parasites.

Test the effectiveness of insecticides to resist external parasites.

Examination of stool samples for internal worms, their eggs and primary parasites.

Make a stool farm to diagnose the stages of nematode and pulmonary worms.

Examination of smears from the intestines of animals for internal and primary parasites.

Examination of water and vegetable samples for primary parasites

Examination of internal organ samples.

Examination of stool samples for primary parasites, such as coccidiosis and antibes.

Examination of aborted fetuses, their entrails, surrounding membranes, serum, toxoplasma and neosporum.

Examination of meat samples to detect sarcocyst sacs.

Examination of tissue samples to detect Leishmania parasite.

Biochemistry Research

Biochemistry, Toxicology and Nutritional Deficiency Research Department

Specializes in the following:

Conduct field research on toxins and new veterinary medicines, study their impact and recommendations for safe use.

It specializes in analyzing veterinary medicines of all kinds (antibiotics - anti-inflammatory - anti-parasitic drugs) in order to determine the ratio of the active substance and its conformity with the specifications.

Detecting chemical toxins and mycotoxins.

Estimate the proportions of heavy metals (cadmium - lead - manganese - copper - nickel - zinc - iron) in water samples, fodder and various tissues.

Pesticide registration and conformity to specifications.

Determination of the half-lethal dose of different pesticides.

Detection of strychnine and phenylphenol.

Detecting fatty acids, total and specific fats in all biological fluids and tissues.

Detecting vitamins of all kinds in biological fluids, tissues and food additives (complementary foods).

Detecting enzymes and hormones of all kinds in biological fluids, tissues and complementary foods.

Detection of mineral salts in biological fluids.

Detection of harmful residues deposited from food packaging and packing materials.

Quantitative and qualitative estimation of carbohydrates in biological fluids and tissues.

Examination of biological preparations and immunizations with a pyrogen test to ensure their safety and suitability for safe use.

Protein electrolysis.

Measuring the level of antioxidants in the blood

Food Hygiene Research

Food hygiene Research Department

Specializes in the following:

The department performs tests on the microbes that cause food poisoning during the detection of microbes (bacteria - fungi - yeasts) that lead to food contamination and spoilage and microbial food poisoning.

The laboratory provides an integrated service of microbiological and chemical tests and examinations and detection of harmful food residues through specialized laboratories for monitoring and industry to ensure their conformity with the requirements and standards in order to grant certificates for conforming to the specifications of good health industrial practices GMP, GHP and the foundations of the HACCP and ISO2000 foodstuff system.

Providing technical advice on precautions and recommendations to be observed to reduce pollution and methods for controlling microbial contamination to ensure the safety and health of the food handled.

The factory provides a specialized team that withdraws food samples from their production and circulation sites according to the international standard methods, for the bodies that wish to use the laboratory and it also provides cars equipped to transport samples to the laboratory.

Virology Research

Virology Research Department

Specializes in the following:

Participation in calibration processes for local and imported biological preparations.

Virus Virology Classification and Classification Research Unit:

Isolating pathogenic viruses and field problems of a large sector of livestock in experimental animals and fertilized eggs, during histological isolation and classification procedure using the most recent and accurate serological tests such as AGPT / VNT / ELISA / CFT / IP / FAT.

Initial, rapid and accurate detection of virus antigens that cause health problems, and rapid identification of them so that appropriate precautions can be taken to prevent diseases.

Titration of various viral strains, laboratory isolated, and comparison with reference.

Keeping isolated laboratory viruses and classifying them as a nucleus to make the necessary vaccines with high efficiency

Serological unit

Serology module (viruses)

It specializes in the following:

Determining the immune bodies and the immune level of the immunized animals to demonstrate the efficacy of the immunization and determining the appropriate time for re-immunization.

Detecting the normal infection by identifying anabolic protein in some viruses such as FMD - blue tongue and others.

Detection of antibodies to the cow's carcinogen virus to examine animal missionaries from the tithe wheels and prove free of disease.

Viral horse disease screening.

Detection of rabies immune bodies for exported and imported domestic animals and proof of vaccine efficacy.

Detecting modern injuries using detectors and identifying them, especially on the common borders of Egypt.

Preparation of reagents, immune bodies and products such as (Rift Valley Fever - lumpy skin - smallpox (camels - sheep - goats)

Bacterial Bacteria Bank

Serology and Bacterial Bank

It specializes in the following:

Isolating salmonella microbe from different sources (feed, feed additives, pet foods, fish meal, meat and bone meal, animal protein powder, beet meal, poultry slaughter powder, evaluation of the efficacy of antiseptics on salmonella microbe).

Biochemical tests and serological classification of Salmonella microbe.

Serological classification of the coli microbe

The various isolates were received from the researchers at the institute and its sub-laboratories for bacteriological examination and then kept in the bacterial bank of bacteria.

Providing the different strains for researchers through the bacterial strains bank.

Buffalo diseases research

Buffalo Disease Research Department

Specializes in the following:

Developing and implementing preventive and curative plans and programs to protect animal wealth from diseases.

Diagnosis of animal diseases in farms and laboratory.

Protecting livestock from incoming diseases through applying border quarantine measures to animals.

Veterinary health supervision of livestock projects and technical advice in the field of animal health.

Evaluating the efficacy and safety of feed and feed additives imported from it, local and the materials involved in their production.

Contribute to preserving public health through setting and implementing programs to control and control common diseases.

Carry out research, preparation and studies related to animal health and public health, or coordinate with other research authorities.

Pathology Research

Pathology Research Department

Specializes in the following:

Create a complete blood picture for different animals and birds.

Microscopic examination of the bone marrow.

Biological fluid examination.

Urine analysis.

Diagnosis of various animal diseases through pathological examination according to the methods of the World Organization for Animal Health and the diagnosis of spongiform cerebral disease (mad cow).

ElISA Research Unit

ElISA` Research Unit and the Viral strains Bank

It specializes in the following:

The unit prepares viral isolates and studies the genetic sequence in cooperation with the Genetic Analysis Unit. And keep them inside the bank of viral doses for use in the production of vaccines.

Biotechnology research

Biotechnology Research Department

Specializes in the following:

Development and conduct of biotechnology research aimed at developing livestock, poultry and fish resources.

The use of modern techniques in the diagnosis of animal, poultry and fish diseases, whether parasitic, bacterial, fungal or viral, using traditional chain reaction polymerization techniques and real-time use of hybridization methods, as well as analysis of different proteins using electrophoresis methods.

The work of testing the DNA and distinguishing the different types of animals in meat and meat products, fodder, waste and plants of animal origin.

Conducting advanced immunological studies using immunological printing methods.

Partial biological studies are undertaken to identify genetic differences between isolates.

Conducting antibiotic resistance gene detection tests.

Doing multiple tests to reveal more than one microbe or discover and classify the microbe at the same time.

Carrying out specialized research in combating external parasites, especially ticks, by analyzing and preparing sexual attractants (pheromones) and making traps from them.

The use of modern techniques in classifying some strains of pathogens.

Use the DNA randomization test to compare isolates and determine epidemiological relationships between them.

Immunology Research

Immunity Research Department

Specializes in the following:

Study the immune response of farm animals after the current vaccinations.

Prepare multicellular immune bodies against local isolates.

Preparing single-cell immune bodies.

Study the effect of immune stimuli on the immune system to improve the immune performance of farm animals, birds and fish.

Study the effect of bacteria, viruses, parasites and inhibitors on the immune system of farm animals, birds and fish.

Study the climatic effects and environmental pollutants on the immune system.

Sub-laboratory research

Sub Laboratories Research Department

The sub-laboratories are considered units for early warning and rapid forecasting of the emergence of epidemic diseases in animals, poultry and fish, knowing that all the previous specializations of the institute are carried out by the sub-laboratories at the level of the Republic.

The department belongs to thirty-two sub-factories distributed all over the country to provide fast service to the public.

Reference Laboratory for Veterinary Control of poultry production

Reference Laboratory for Veterinary Control of poultry production

Established the National Laboratory for Veterinary Control of Poultry Production in 2003 within the Animal Health Research Institute. The National Laboratory is the only entity competent to diagnose bird flu and monitor and evaluate the immunizations related to the disease in the field. The laboratory was approved in 2005 by the ISO 17025 specifications by an evaluation team from the National Office for the accreditation of laboratories recognized by the International ILAC and it was ensured that the quality system in the laboratory conformed to the administrative and technical requirements and use The latest standard methods of testing and the latest laboratory equipment and their calibration to ensure accuracy of measurement as well as to ensure the efficiency of personnel working as the laboratory provides the appropriate environment to perform the tests

Duties and services

The factory undertakes veterinary control work on poultry production to ensure that there are no health problems of economic importance and in a manner that meets the standards of standard quality and bio-security, and for this purpose:

Examination of eggs from poultry and chickens origins, a day's life from ancestral herds, laying mothers and imported and locally produced meat mothers, and issuing health certificates in accordance with local and international standards

Applications are submitted to the National Laboratory for Veterinary Control of Poultry Production to examine samples of poultry goods to be exported and who performs the necessary checks in accordance with international standards and protocols

Carrying out periodic inspection of poultry projects with all their activities to ensure that they are free of pathogens at time intervals consistent with the productive cycle of each activity (ancestral herds, white mothers, broiler mothers, broiler herds, hatcheries and laboratories attached to these activities) and issuing health reports

Examination of foods of poultry origin of pathogens

Solve field problems facing the poultry industry in Egypt and provide technical advice through field visits to discover the pathogens

Future vision for the Reference laboratory

Attention to common diseases between humans and birds by training technical and research cadres in the field of laboratory diagnostics and conducting epidemiological studies and preventive medicine and setting the necessary procedures to limit the spread of these diseases.

The optimal use of infrastructure from equipped laboratories that can serve all specialties in order to provide laboratory services for all units

Developing administrative systems (human resources - purchases - supportive technical services - stores) in order to provide the appropriate environment for the completion of works and to preserve the available buildings, devices, means of transportation, etc.

In the context of the efforts exerted by the Reference Laboratory of the Ministry of Agriculture to reduce the imminent danger to human and poultry health from the transmission of bird flu infections and to prepare for and control the spread of influenza and other contagious diseases among individuals and in order to develop the performance of the Reference laboratory to carry out the tasks assigned to it, it was necessary Create an integrated database for the Reference laboratory and its branches in the governorates

Genome Research Unit

Genome Research Unit

• It specializes in the following:

Genetic analysis of Egyptian isolates, whether bacterial, viral, parasitic or fungal, for classification

And knowledge of virulence genes with it.

• Developing research based on gene expression and reverse genetics.

Make a comparison between modern isolates and their counterparts used in Egyptian vaccines to update

Vaccines.

• Contributing to the production of diagnostics and developing vaccine production from local strains, on the basis of principles

Genetic

• Development of the bacterial and viral strains bank on genetic basis

Conducting complete genome projects for Egyptian bacterial, viral, parasitic or fungal isolates.

Rapid diagnosis of diseases based on the technique of protium or genome microarray.

• Doing a pedigree in horses and other animals.

Brucellosis Research

Brucellosis Research

Ministry of Agriculture & Land Reclamation

Agricultural Research Centre
Animal Health Research Institute

Diagnostic Activities of Brucellosis Research Department

Animal Health Research Institute, Dokki, Giza 12618, Egypt

Activities of Brucellosis Department

The Department of Brucellosis embraces three units:

A.  Unit of Brucella Isolation, Identification & Typing

This unit is concerned with the bacteriological diagnosis of brucellosis in different animals.

·        Isolation of the causative agent from aborted foeti, lymph nodes, spleen, liver, milk……etc.

·        Recognition of isolates as belonging to genus Brucella by colonial morphology, microscopic appearance, test for dissociation, agglutination with Brucella antisera, CO2 requirement, test for motility, culture onto blood and MacConkey agar, catalase test, oxidase test, urease test, nitrate reduction test and glucose fermentation test.

·        Identification to the Brucella species level by multi-phage lysis tests

·        Complete typing of Brucella to the biovar level by CO2 requirement, H2S production, sensitivity to dyes and agglutination with monospecific antisera.

·        Evaluate the efficacy of disinfectants on Brucella organisms.

·        Differentiate Between Brucella Vaccinal strains (RB51, S19, & Rev1) and field Brucella strains.

·        Brucella Detection & Identification by PCR:

-         Extraction of Brucella DNA

-         Application of PCR for identification of Brucella on Genus level.

-         Application of AMOS-PCR for identification of Brucellae on biovar levels.

B.   Unit of Brucella Serology

This unit is concerned with the serological diagnosis of brucellosis in different animals.

§  Rapid screening techniques including the buffered acidified plate antigen (BAPAT), brucellosis card (Rose Bengal – RBT) and milk ring tests (MRT).

§  Supplementary techniques involving the serum tube (or plate) agglutination, mercaptoethanol, rivanol-precipitation and complement fixation tests and ELISA techniques.

§  Monitoring Post-vaccinal immune response in vaccinated animals.

 C.   Unit of Milk & Milk Product Examination for Brucella

This unit is concerned with the antibodies detection and bacteriological examinations of milk and milk products :

Serological examination of milk by the milk ring test (MRT) & iELISA

Bacteriological examination of milk & dairy products for Brucella

Diagnostic Tests of brucellosis

Sampling

-         Aborted foeti             - Foetal membranes

 -         Tissues & L.N.          - Vaginal swabs              - Blood for culture

Bacteriological examination for brucellosis:

1)    Isolation of the causative agent

a-     By direct culture onto solid media

b-    By animal inoculation

2)    Recognition of isolates belonging to the genus Brucella

1.     Colonial morphology:

2.     Microscopic appearance:

3.     Test for dissociation:

4.     Agglutination with Brucella antisera:

5.     CO2 requirement:

6.     Test for motility:

7.     Culture onto blood and MacConkey agar:

8.     O2 requirement:

9.     Catalase test:

10.     Oxidase test:

11.      Nitrate reduction test:

12.     Glucose fermentation test:

 Identification to the Brucella species level by phage lysis

 -         Firenze (Fi)                - Weybridge (Wb)                    -Berkley (Bk2)

-         R/ canis (R/C)            - Izatnagar (Iz1)

3)    Complete typing of Brucella to the biovar level

-         CO2 requirement H2S production - Urease test - Sensitivity to dyes  - Agglutination with polyclonal monospecific antisera

Serological Diagnostic Tests

Sampling

-         Blood sera

-         Seminal plasma

-         Milk serum (whey)

 Serological tests for brucellosis

1.     Serum agglutination test

2.     Mercaptoethanol test

3.     Buffered acidified plate antigen test

4.     Brucellosis card test (BCT or Rose-Bengal test)

5.     Rivanol-precipitation plate agglutination test

6.     Complement fixation test

7.     Indirect ELISA on Serum

8.     Indirect Milk ELISA

9.     Competitive ELISA

Examination of milk & milk products

Sampling

-        Milk serum (whey)

-        Milk for culture

-        Cheese, Youghurt & Butter

    -        Milk ring (MRT) or abortus Bang ring (ABR) test

Bacteriological examination of milk & its products for brucella

1)    Isolation of the causative agent

¨    By direct culture onto solid media

¨    By animal inoculation

2)    Identification to the Brucella species level by phage lysis

3)    Complete typing of Brucella to the biovar level

Training courses

Training course for Iraqis under the title of seroprevalence, diagnosis, and control programs of brucellosis, was held in Muscat, Oman, during the period from 6 to 10th November 2016. This training course was done under the supervision of both AHRI and the Arab Organization of Agricultural Development (AOAD). 

Training course for Middle East veterinarians under the title of seroprevalence, diagnosis, and control programs of brucellosis, was held in Muscat, Oman, during the period from 13 to 17th November 2016. This training course was done under the supervision of both AHRI and the Arab Organization of Agricultural Development  AOAD

In a continuous and fruitful co-operation with the Arab Organization of Agricultural Development (AOAD), a training course to Sudanese veterinarians under the field of diagnosis and control programs of brucellosis held in Khartoum, Sudan during the period from 7 to 11th August 2016.

A training course in the field of diagnosing transboundary animal diseases and the role of veterinary quarantines in controlling them and their impact on trade, from 14-18 / 1/2018

This course was held at the Brucellosis Research Department - Animal Health Research Institute in cooperation with the Ministry of Foreign Affairs

Training course in the management of quarantine facilities and laboratory diagnosis of trade limiting diseases held in the Animal Health Research Institute, Cairo, Egypt in the period from 29th October to 6th November 2016

(This program was under the supervision of the African Union,  Bureau from Animal Resources (AU-IBAR)

Airport Veterinary Laboratory